Differential Reference Electrode Applied to Arrayed pH Sensor and Arrayed Glucose Biosensor Based on Microfluidic Framework

In this study, the radio frequency (R. F.) sputtering system and screen printing technology were used to fabricate the arrayed pH sensor. And the glucose oxidase (GOx) enzyme was immobilized on the arrayed pH sensor as the arrayed glucose biosensor. Furthermore, the computer numerical control (CNC) technique was used to fabricate the microfluidic mold. The differential reference electrode can provide a stable potential in different pH solutions, which was applied in arrayed pH sensor and arrayed glucose biosensor. The arrayed pH sensor and arrayed glucose biosensor were integrated to the poly-dimethylsiloxane (PDMS) microchannel as the microfluidic device for observing the characteristics of the arrayed pH sensor and arrayed glucose biosensor at dynamical conditions. At dynamical conditions with flow rate controlled at 20 ml/min, the average sensitivity and linearity of the arrayed pH sensor were 57.27 mV/pH and 0.986, respectively. And at the flow rate controlled at 5 ml/min, the average sensitivity and linearity of This study has been supported by National Science Council, Republic of China, under the contracts NSC 101-2221-E-224-046, NSC 101-2221-E-265-001, NSC 100-2221-E-224-017, NSC 100-2221-E-268-004 and NSC 97-2221-E-224-058-MY3. Tsung-Yi Cheng is with the Graduate School of Electronic and Optoelectronic Engineering, National Yunlin University of Science and Technology, Douliou, Taiwan 64002, R.O.C. (e-mail: [email protected]). Jung-Chuan Chou is with the Graduate School of Electronic and Optoelectronic Engineering, National Yunlin University of Science and Technology, Douliou, Taiwan 64002, R.O.C. (corresponding author to provide phone: (886)5342601 ext.4333; fax: (886)5312063; e-mail: [email protected]). Yi-Hung Liao is with the Department of Information Management, TransWorld University, Yunlin, Taiwan, R.O.C. (e-mail: [email protected]) Ya-Li Tsai is with the Department of Electronic Engineering, National Yunlin University of Science and Technology, Douliou, Taiwan 64002, R.O.C. (e-mail: [email protected]). Jyun-Wun Lin is with the Graduate School of Electronic and Optoelectronic Engineering, National Yunlin University of Science and Technology, Douliou, Taiwan 64002, R.O.C. (e-mail: [email protected]). Chiung-Yun Jhang is with the Department of Electronic Engineering, National Yunlin University of Science and Technology, Douliou, Taiwan 64002, R.O.C. (e-mail: [email protected]). Chia-Yu Liu is with the Graduate School of Electronic and Optoelectronic Engineering, National Yunlin University of Science and Technology, Douliou, Taiwan 64002, R.O.C. (e-mail: [email protected]). Jui-En Hu is with the Graduate School of Electronic and Optoelectronic Engineering, National Yunlin University of Science and Technology, Douliou, Taiwan 64002, R.O.C. (e-mail: [email protected]). Hsueh-Tao Chou is with the Graduate School of Electronic and Optoelectronic Engineering, National Yunlin University of Science and Technology, Douliou, Taiwan 64002, R.O.C. (e-mail: [email protected]). the arrayed glucose biosensor were 9.67 mV(100 mg/dL) and 0.943, respectively.